Sensitive Assay Design For Detection Of Anti Drug Antibodies To Biotherapeutics That Lack An Immunoglobulin Fc Domain Scientific Reports
Development of Pharmacokinetic (PK) Assays for Detecting Biosimilars Targeting TNFa Using AlphaLISA Introduction Biotherapeutic proteins, such as monoclonal antibodies, have proven to be highly effective as drugs Antibodies against these drugs (antidrug antibodies) can be utilized in assays to detect the drugs in patient samplesThe enzyme linked immunosorbent assay (ELISA) is a powerful method for detecting and quantifying a specific protein in a complex mixture Originally described by Engvall and Perlmann (1971), the method enables analysis of protein samples immobilized in microplate wells using specific antibodies ELISAs are typically performed in 96well or 384
